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Phytochemical, antimicrobial and anthelmintic studies on leaves of ficus hispida Linn. Synopsis for


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PHYTOCHEMICAL, ANTIMICROBIAL AND ANTHELMINTIC STUDIES ON LEAVES OF Ficus hispida Linn.


SYNOPSIS FOR

M.PHARM DISSERATION

SUBMITTED TO


RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES
BY
Dhruva kumar

Department of Pharmacognosy




THE OXFORD COLLEGE OF PHARMACY

Bangalore -78




RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,

KARNATAKA,BANGALORE.

ANNEXURE-II




PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION





1

Name of the candidate and address

DHRUVA KUMAR


s/o – Mr. RAM CHARAN,

20/17, Mori Daraganj,

Allahabad, Uttar Pradesh-211006




2


Name of the institution

The Oxford College Of Pharmacy,

J.P Nagar,1st Phase,

Bangalore 560078



3


Course of study and subject

MASTER OF PHARMACY

(PHARMACOGNOSY)




4


Date of the admission

12th JUNE 2008






5



Title of the topic:







PHYTOCHEMICAL, ANTIMICROBIAL AND ANTHELMINTIC STUDIES ON LEAVES OF Ficus hispida Linn.






6


7.



Brief resume of the intended work

6.1 Need for the study

The plant Ficus hispida linn belonging to the family Moraceae, also called as Katguleriya, is distributed throughout the outer Himalayas from Chenab eastwards to Bengal, Central South India and Andaman Islands. It generally occurs in shady places and along ravines.

The leaves of Ficus hispida contains bergapten, β amyrin and β-sitosterol. The bark of Ficus hispida contains N-triacontanyl acetate, gluacolacetate, 10- keto tetracosyl arachidate and lupeol acetate. Also other phytoconstituents like phenanthroindolizine, gluacol acetate, biphenylhexahydroindolizine alkaloid are also isolated from Ficus hispida1.

The methanolic extract of the leaves of Ficus hispida has been reported to be hepatoprotective2, anti-diarrhoeal in rats3. The ethanolic extract of Ficus hispida has been reported for anti-oxidant4, analgesic activity5.

So far various studies have been carried out with respect to various plant parts for different pharmacological activity, but no data is there to established certain traditional uses mentioned for Ficus hispida like anthelmintic, anti-fertility etc.

So the present studies have been taken up to establish / provide scientific data for some of these activities.



Therefore in the present study it is proposed to carry out isolation of the chemical constituents from leaves of Ficus hispida, antimicrobial and anthelmintic activity of the extracts and / isolated compounds.



6.2 Review of literature

  • β-amyrin acetate lupeol acetate and β-sitosterol was reported from the bark while β-sitosterol, β-amyrin, β-soralen and bergapten were reported from the leaves of the Ficus hispida1.

  • The methanolic extracts of leaf Ficus hispida shows hepatoprotective activity against paracetamol induced hepatotoxicity in rats2.

  • Methanolic extracts of Ficus hispida leaf showed significant inhibitory activity against castor oil induced diarrhoea and PGE­2 induced enteropooling in rats3.

  • Ethanolic extract of the bark of Ficus species showed antioxidant activity by DPPH method4.

  • The ethanolic extract of Ficus hispida shows significant analgesic activity in mice5.

  • A new norisoprenoid, ficustriol and the known phenanthroindollizine alkaloid o-methylophlorinidine, were isolated from CHCl3 extract of the leaves and twigs of Ficus hispida. O-methyl tylophoorinidine showed potent cytotoxic activity when tested against a small panel of human cancer, while ficustriol was inactive6.

  • The leaves extract of Ficus hispida showed the presence of 3,6,7 trimethoxy phenanthroendolizidine and 3,6,7- trimethoxy 14-hydroxy phenanthroendolizidine The cholorofrom extract of Ficus hispida fruit showed positive effect against the inflammation in carragenin induced rat hind paw oedema and fall of blood pressure in anethesthesized cats7.

  • Hispidine was isolated and charecterised from the fresh leaves extract of Ficus hispida8.

  • Extracts of the various parts of Ficus hispida were found effective in the treatment of wound healing and jaundice9.


6.3 Objectives of the study:
The objective of the present studies is to collect the drug, that is leaf of Ficus hispida from Gulbarga(karnataka) authenticated by CCRAS Bangalore. To carry out the extraction (maceration/ soxhlet method) and isolation of the phytoconstituents from selected extract based on phytochemical screening and further characterization of isolated compound by spectral analysis( UV,IR and NMR spectroscopy). The different extracts and / or isolated compound would be studied for antimicrobial activity (by agar diffusion method) and anthelmintic activity10 (by using earth worm Pheretima posthuma).



7.1 Materials and methods:


  1. Sources of data




  • Chemical abstracts

  • Medline

  • Other Journal from the oxford college of Pharmacy.

  • RGUHS digital library


b) Place of work

The oxford College of Pharmacy, Bangalore.





7.2 Method of collection of materials


  • Collection: - The samples of leaf of Ficus hispida Linn collected from Gulbarga (Karnataka) and authenticated from CCRAS, Bangalore.

  • Extraction: - Extract of the drugs will be prepared using different solvent by maceration/ soxhlet.

  • Phytochemical Screening:-The extract would be subjected for preliminary phytochemical screening for various phytochemical groups.

  • Isolation: - Isolation of extracted phytoconstituents will be done using column chromatography /separation method.

  • Identification and characterization: - The isolated compound will be identified using analytical methods like UV, IR, and NMR spectroscopy.

  • Antimicrobial activity: - The extract and/or isolated compound will be studied for anti- microbial activity (by agar diffusion method).

  • Anthelmintic activity: - The extract and/or isolated compound will be studied for Anthelmintic activity (by using earth worm Pheretima posthuma) 10.





7.3 Does the study require any investigation or interventions to be conducted on

patients or other humans or animals?

Not applicable.




7.4 Has ethical clearance been obtained from your institution in case of 7.3?
Not applicable






8.


Bibliography


  1. Sharma PC, Yelne MB, Dennis TJ. Data base on medicinal plants used in Ayurveda Central Council for Research in Ayurveda and Siddha New Delhi. 2002;(5):96.

  2. Mandal SC, Saraswathi B, Ashok kumar CK, Mohana Lakshmi S, Maiti BC. Protective effects of leaf extract of Ficus hispida Linn. against paracetamol- induced hepatotoxicity in rats. Phytotherapy Research. 2000; 14(6):457- 459.

  3. Mandal SC, Ashok Kumar CK. Studies on anti-diarrhoeal activity of Ficus hispida leaf extract in rats. Fitoterapia . 2002; 73(7-8):663-667.

  4. Kalkar S, Kshirsagar NV, Paulkar NM. Antioxidant power of phytochemicals from Ficus species in relation to its phenolic content. International Conference on Modern Trends in plant Sciences with Special Reference to the Role of Biodiversity in Conservation. 2005; 90.

  5. Chandrasekhar Rao M, Ravi Kumar A, Mayuren C. Analgesic activity of Ficus hispida linn. International journal of chemical sciences. 2007; 5(3):1225-1228.

  6. Peraza Sanchez S, Chai HB, Shin YG, Santisuk T, Reutralkul V, Farnsworth et.al. Constituents of the leaves and twigs of Ficus hispida. Planta Medica. 2002; 68(2):186-188.

  7. Khare CP. Encyclopedia of Indian Medicinal Plant-Rational, Western Therapy. Ayurvedic and other traditional usage Botany, Springer-Verlag Berlin Heidelberg. 2004; 220.

  8. Rastogi RP, Mehrotra BN. Compendium of Medicinal Plants, Central Drug Research Institute, Lucknow and National Institiute of Science Communication, New Delhi. 1980; (3):294.

  9. Compendium of Medicinal Plants by National Institute of industrial Research Board of consultants and Engineers New Delhi.125.

  10. Dash Gk, Mishra B, Panda A, Patro CP, Gagapaty S. Anhelmintic activity of Evolvulus nummularius. Indian journal of natural product. 2003; 19(3):24-25.

9.


Signature of Candidate




10.

Remarks of the Guide



Recommended


11.

11.1 Name and Designation of Guide



Mrs.Usha Gavani

Asst.Professor

Department of Pharmacognosy





11.2 Signature








11.3 Co-Guide








11.4 Signature








11.5 Head of the Department


Dr. Padmaa M. Paarakh


Professor and Head

Department of Pharmacognosy






11.6 Signature





12.

12.1 Remarks of the Chairman and

Principal



Forwarded to the University for scrutiny




12.2 Signature


Dr. Padmaa M. Paarakh

Principal

The Oxford College of Pharmacy,



J.P.Nagar, Ist Phase, Bangalore – 78









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