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Hristo Najdenski, PhD, Associate Professor


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Hristo Najdenski, PhD, Associate Professor

Head of Department

Tel: (+359 2) 979-31-61

E-mail: usema@bas.bg

1958 - Born October 24, Velingrad

1982 - Diploma, Faculty Veterinary Medicine, Stara Zagora

1987 - PhD in Micribiology,The Stephan Angeloff Institute of Microbiology, BAS

1988 - Assistant Professor The Stephan Angeloff Institute of Microbiology

1992-93 - Specialization in the Institut Pasteur, Paris, France

1996 - Associate Professor, The Stephan Angeloff Institute of Microbiology

2000-today - Head of the Department Pathogenic Bacteria and Deputy Director of the Stephan Angeloff Institute of Microbiology

2002 – Specialization in the Department of Medical Biochemistry, Turko University, Finland

2005 – Specialization in the Institute for Agricultural and Fisheries Research, Technology and Food Unit, Melle, Belgium
Tatyana Radoucheva, PhD, Assoc. Professor

Head of Research Team

Tel: (+359 2) 979-31-68

E-mail: tradoucheva@abv.bg

1945 – Born July 31, Sofia

1968 – Diploma, Faculty of Biology, Sofia State University

1977 – PhD in Micribiology, The Stephan Angeloff Institute of Microbiology, BAS

1979 – Assistant Professor The Stephan Angeloff Institute of Microbiology

1987 – Associate Professor, The Stephan Angeloff Institute of Microbiology

1994-2000 – Head, Department of Pathogenic Bacteria, The Stephan Angeloff Institute of Microbiology, BAS


Nadya Markova, PhD, Assoc. Professor

PhD – The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences

MD – Pleven Medical University

Head of Research Team

Tel: (+359 2) 979-31-68

E-mail: nadya.markova@gmail.com


Vesselin Kussovski, PhD, Assoc. Professor

Head of Research Team

1975 - Diploma, Faculty of Biology, Sofia State University

1986 - PhD – The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences

2005 - Associate Professor, The Stephan Angeloff Institute of Microbiology

Tel: (+359 2) 979-31-68

E-mail: vkussovski@yahoo.com
Anna Vesselinova, Dr. Sc., Professor

E-mail: MStoy@abv.bg


Neli Slavova-Azmanova, PhD, MD

E-mail: nellystz@yahoo.com


Elica Golkocheva-Markova, PhD

E-mail: golkocheva@microbio.bas.bg


Mariana Ninova, MSc

E-mail:
Neli Georgieva, DVM

E-mail:
Iva Tzvetkova, MSc

E-mail: likovska@abv.bg


Anton Caro, DVM

E-mail: anton_caro@abv.bg


Mihail Iliev, PhD-student

E-mail: Iliev@microbio.bas.bg


Violeta Valcheva, PhD-student

E-mail: vivi088@abv.bg


Albena Cherneva

Radka Georgieva
Main Research Topics:

  • Factors and mechanisms of bacterial virulence

  • Bacterial-host interactions in zoonoses and particularly dangerous infections

  • Surface bacterial structures and bioenergetic mechanisms

  • Sunlight and the survival of Enterobacteriaceae bacteria in water ecosystems

  • Influence of environmental factors on the survivability of pathogenic yersiniae - epidemiology and prevention.

  • Detection and enumeration of pathogens in products of animal origin by using the polymerase chain reaction.

  • In vitro determination of antibacterial and antifungal activity of substances with organic and non-organic origin.

  • Photophysical and photosensitizing properties of phthalocyanine complexes as antibacterial and antiviral agents

  • Molecular characterisation of Mycobacterium tuberculosis strains and Monitoring the burden and transmission of tuberculosis in Bulgaria; Incidence of multidrug-resistant TB (MDR-TB); Genotypic detection of drug resistant mutations



Most Important Achievements:

  • The increased growth ability and virulence of septicaemic pathogens in animal corps was found, and the relationship of this phenomenon to the changes in energetic metabolism of the bacteria was proved.

  • Pathogenic strains of the species Yersinia enterocolitica have been isolated from children with gastrointestinal disorders, from tonsils of pigs, as well as from the viscera of wild animals (boars, wild cats, deers, birds, and fishes). Microorganisms of the species Yersinia pseudotuberculosis (serotypes 0:1, 0:2, 0:3, and 0:5) have been isolated from the viscera of boars, wild cats and birds.

  • Yersinia outer membrane proteins have been isolated and used in improved ELISA and immunoblot tests for routine laboratory diagnosis of natural yersiniosis in humans and pigs and experimental yersiniosis of rabbits.

  • Bacterial-host interactions (invasion, persistence, intracellular survival, as well as the bioenergetic mechanisms ensuring these processes) were characterized. Pathogenesis of experimental melioidosis, listeriosis, yersiniosis, salmonelosis, etc. and immune response of different hosts were established in details.

  • Clinical, immunological, and pathomorphological examinations were followed after experimental oral infections of rabbits with two wild and four genetically constructed mutant strains of Y. enterocolitica 0:8. A lower virulence and bacterial load of the viscera, weaker morphological changes, better immunogenicity and well expressed tissue tropism to the lymphoid tissue of the mutant strains DwzzGB and sodA-314 in comparison with mutants 8081-R2, DwbcEGB and wild strains WA-314 and 8081-L2 were established.

  • The photoinhibitory effect could be used in the practice for photosterilization of contaminated with enterobacteria water ecosystems;

  • The role of Yersinia antigens (LPS) in induction of reactive arthritis was proved in experimental models.

  • The role of outer membrane proteins YopK and YpkA during experimental oral pig model with Yersinia pseudotuberculosis was studied. It was established that these mutant strains are less virulent in comparison with wild type strain. Nevertheless the strains were characterized with high immunogenic potential and ability to colonize the lymphoid tissues. This ability was the highest in yopK mutant strain.

  • The first time in our country was studied the incidence and transmission of multidrug-resistant Mycobacterium tuberculosis strains using advanced molecular epidemiological methods and population structure analysis. The multidrug resistant and hypervirulent Beijin strain was not found in Bulgaria. The results contribute to control the global spread of multidrug TB worldwide.

  • It was found that the methicilin-resistant and methicilin-sensitive strains of Staphylococcus aureus as well as Pseudomonas aeruginosa and Candida albicans can be effectively photoinactivated with the cationic phthalocyanine ZnPeMe. Anionic phtalocyanine ZnPcS and cationic phthalocyanine ZnPeMe are effective virucidal photosensitisers for the studied DNA-viruses – Herpes virus simplex type I and Vaccinia virus.

  • The method of PCR-DGGE was optimized and successfully applied for direct detection and typing of Campylobacter jejuni and Campylobacter coli in caecal samples of broilers without preliminary enrichment. This method allows reliable and fast detection and identification of pathogenic Campylobacters being causative agent №1 of food infections in humans.


Projects

National
Virulence factors of Yersinia and their application

Project leader: H. Najdenski, PhD

Grant К-1406/2004 from the National Scientific Research Council, Republic of Bulgaria
Characterization of physicochemical and catalitical properties of bacterial uridine monophosphate kinases

Project leader: N. Slavova-Azmanova, MD,

Grant MU-К-1406/2004 from the National Scientific Research Council, Republic of Bulgaria
Incidence and nature of the phenomenon “heteroresistance” in methicilin resistant staphyloc occi

Project leader: N. Markova, PhD

Grant 19/2004. Contract research agreement between Medical University of Plovdiv and Institute of Microbiology, BAS, Republic of Bulgaria
Photodynamic inactivation of pathogenic bacteria and viruses by phthalocyanines: ecological and medical aspects

Project leader: V.Kussovski, PhD

Grant B-1534/05 from the National Scientific Research Council, Republic of Bulgaria
International

Optimization of molecular typing methods for Campylobacter

Project leader: H. Najdenski Advisors: L. Herman, PhD, V. De Jonghe, PhD

Grant of the Belgium Ministry of Science and Education


Physicochemical and biochemical studies of recombinant bacterial enzymes involved in nucleoside metabolism

Project leader: N. S. Slavova-Azmanova, MD,

Grant RIG.981473 from NATO Security through Science Program
Intervention strategy against the development of multidrug-resistant tuberculosis during therapy

Project leader: N. Markova, PhD

Grant NATO EAP.CLG.980734 TBC/2004-2006
Molecular epidemiology of tuberculosis in Bulgaria

Project leader: N. Markova, PhD

Contract research agreement between St. Petersburg Pasteur Institute, Russia and Institute of Microbiology, BAS, Bulgaria
Application in clinical proteomics in Yersinia diagnosis

Project leader: E. Golkocheva, PhD

Advisors: H. Najdenski, PhD, R. Stoilov, PhD

Grant RIG.981256 from NATO Security through Science Program


Selected Publications (2000-2006) :

Kamenarska, Z., S. Konaklieva, C. Nikolova, A. Kujumgiev, K. Stefanov, S. Popov. Volatile components of the freshwater algae Spirogyra and Mougeotia. Z. Naturforsch, 2000, 55, 495-499.

Najdenski, H., Y. Michailov, S. Nikolova, A. Vesselinova. In vitro study of antiyersiniosis effect of Oxadin. Die Pharmazie, 2000, 55, 7, 548-549.

Najdenski, H., A. Vesselinova, S. Nikolova. Ecological, epidemiological and clinical aspects of yersiniosis. Infectology, 2000, 37, 2, 6-9.

Najdenski, H., E. Golkocheva, P. Neykov, A. Vesselinova. Serological screening of Yersinia infections in pigs by the classical agglutination test and improved ELISA. Proceedings 16th Congress of the International Pig Veterinary Society, Melbourne, Australia, 2000, 241.

Nikolova, S., D. Wesselinova, A. Vesselinova. Pretreatment of guinea pigs with iron or Desferal influence the course of Yersinia enterocolitica infections. J. Vet. Med. B, 2001, 48

Stefanof, I., S.Nikolova, H. Najdenski, A.Vesselinova. Swine tonsils as reservoir for yersiniae. Proceedings 16th Congress of the International Pig Veterinary Society, Melbourne, Australia, 2000, 242.

Veliêova, M., V. Bankova, K. Sorkun, S. Honcine, I. Tsvetcova, A. Kujumgiev. Propolis from the Mediterranean region: chemical composition and antimicrobial activity. Z. Naturforsch, 2000, 55, 790-793.

Velikova, M., V. Bankova, M. C. Marcucci, I.Tsvetcova, A. Kujumgiev. Chemical composition and biological activity of propolis from brasilian meliponinae. Z. Naturforsch, 2000, 55, 785-789.

Vesselinova, A., H. Najdenski, S. Nikolova, P. Neykov. Experimental Listeria monogytogenes infection of pigs. Proceedings 16th Congress of the International Pig Veterinary Society, Melbourne, Australia, 2000, 240.

Najdenski, H.M., E.N. Golkocheva, A.M. Vesselinova. Analysis of pathogenicity markers in wild and mutant strains of Yersinia enterocolitica 0:8. Clin. Microb.& Infection, Suppl.1, 2001, 27-28.

Nikolova, S., Y. Tzvetkov, H. Najdenski, A. Vesselinova. Isolation of pathogenic Yersiniae from wild animals in Bulgaria. J. Vet. Med. B, 2001, 48, 203-209.

Vesselinova, A., H. Najdenski, S, Nikolova, D. Wesselinova. Arthritis after experimental infection with Yersinia enterocolitica 0:3 in rabbits. J. Vet. Med. B, 2001, 48, 43-53.

Markova N., Kussovski V., Radoucheva T., Dilova k., Georgieva N. Effects of intraperitoneal and intranasal application of Lentinan on cellular response in rats. Intern. Immunopharmacol., 2002, 2, 1641-1645.

Najdenski H., Michailov Y., Nikolova S., Vesselinova A. Protective effect of Oxadin on experimental Yersinia enterocolitica infection in rats. Pharmazie, 2002, 57, 337-339.

Najdenski H., Vesselinova A. Experimental mixed infection of rabbits with Yersinia enterocolitica and Listeria monocytogenes. J. Vet. Med. B, 2002, 49, 97-104.

Taskova R., Mitova M., Najdenski H., Tzvetkova I., Duddeck H. Antimicrobial activity and cytotoxicity of Carthamus lanatus. Fitoterapia, 2002, 73, 540-543.

Kamenarska Z., Gasic M.G., Zlatovic M., Rasovic A., Sladic D., Kljajic Z., Stefanov K., Seizova K., Najdenski H., Kujumgiev A., Tsvetkova I., Popov S. Chemical composition of the brown alga Padina pavonia (L.) Gaill. from the Adriatic sea. Bot. Mar., 2002, 45, 339-345.

Kamenarska Z., Dimitrova-Konaklieva St., Stefanov K., Najdenski H., Tsvetkova I., Popov S. Comparative study on the volatile compounds from some Black sea brown algae. 2002, 45, 502-509.

Najdenski H., Vesselinova A., Golkocheva E., Garbom S., Wolf-Watz H. Characterization of infections with wild and mutant Yersinia pseudotuberculosis strains in rabbit oral model. In: The Genus Yersinia. (Edited by M. Skurnik et al.), Kluwer Plenum, 2003, 117-120.

Najdenski, H., Vesselinova, A., Golkocheva, E., Garbom, S., Wolf-Watz, H. Experimental infections with wild and mutant Yersinia pseudotuberculosis strains in rabbits. J. Vet. Med. B, 2003; 50, 280-288.

Najdenski, H., Golokocheva, E., Vesselinova, A., Bengoechea, J-A., Skurnik, M. Proper expression of the O-antigen of lipopolysaccharide is essential for the virulence of Yersinia enterocolitica O:8 in experimental oral infection of rabbits. FEMS Immunol. Med. Microbiol., 2003; 38, 97-106.

De Rosa, S., Kamenarska, Z., Stefanov, K., Dimitrova-Konaklieva, S., Najdenski, C., Tsvetkova, I., Ninova, V., Popov, S. Chemical composition of Corallina mediterranea Areschoug and Corallina granifera Ell. et Soland. Z. Naturforsch., 2003; 58c, 325-332.

Bengoechea J.A., Najdenski H., Skurnik M., Essential role of the lipopolysaccharide O-antigen status of Yersinia enterocolitica O:8 in virulence and in the outer membrane regulatory network. Mol. Microbiol., 2004, 52: 451-469.

Najdenski H., Golkocheva E., Vesselinova A., Russmann H., Comparison of the course of infection of virulent Yersinia enterocolitica serotype O:8 with an isogenic soda mutant in the peroral rabbit model. Int. J. Med. Microbiol., 2004; 294: 383-393.

Ivanova V., Graefe U., Schlegel B., Kolarova M., Aleksieva K., Najdenski H., Tzvetkova I., Chipeva V., Usnic acid, metabolite from neuropogon sp., an antarctic lichen isolation, structure elucidation and biological activity. Biotechnol. & Biotechnol. Eq., 2004; 18: 66-71.

Kamenarska Z., Stefanov K., Dimitrova-Konaklieva S., Najdenski H., Tsvetkova I., and Popov S., Chemical composition and biological activity of the brackishwater green alga Cladophora rivularis (L) Hoek. Botanica Marina, 2004; 47: 215-221.

Najdenski, H., V.Kussovski, A.Vesselinova. Experimental Burkholderia pseudomallei infection of pigs. J. Vet. Med. B 2004; 51: 225-230.

Ignatova M, Voccia S, Gilbert B, Markova N, Mercuri PS, Galleni M, Sciannamea V, Lenoir S, Cossement D, Gouttebaron R, Jerome R, Jerome C. Synthesis of copolymer brushes endowed with adhesion to stainless steel surfaces and antibacterial properties by controlled nitroxide-mediated radical polymerization. Langmuir. 2004; 20 (24):10718-26.

Markova, N., L. Michailova, V. Kussovski, M. Jourdanova, T. Radoucheva. Intranasal application of Lentinan enhances bactericidal activity of rat alveolar macrophages against Mycobacterium tuberculosis. Pharmazie, 2005; 60 (1): 42-48.

Popova M., Bankova V., Naydensky Ch., Tsvetkova I., and Kujumgiev At. Comparative study of the biological activity of propolis from different geographic origin: a statistical approach. Macedonian pharmaceutical bulletin, 2004; 50: 9-14.

Trusheva B., Popova M., Naydensky H., Tsvetkova I., Gregorio Rodriguez J., and Bankova V. New polyisoprenylated benzophenones from Venezuelan propolis. Fitoterapia, 2004; 75: 683-689.

Assairi, L., Bertrand, A., Ferdinand, J., Slavova-Azmanova, N., Christensen, M., Briozzo, P., Craescu, C., Schaeffer, F., Neuhard, J., Barzu, O., Gilles, A.-M. “Deciphering the function of an ORF: Salmonella enterica Deo M protein is a new mutarotase specific for deoxyribose” Protein Science 2004, 13, 1295-1303.

Najdenski H., Rijpens N., Tcherneva E., Herman L., Subtractive hybridization and PCR amplification for detection of Brucella spp. In: Medical aspects of the fight against chemical and biological terrorism and mass traumatism, (ed. Monov A., Dishovsky Ch.), v.1, 2004

Markova N, Michailova L., Kussovski V, Jourdanova M, Radoucheva T. Intranasal application of lentinan enhances bactericidal activity of rat alveolar macrophages against Mycobacterium tuberculosis. Pharmazie. 2005 Jan; 60(1):42-8.

Drandarska, I, Kussovski, V., Nikolaeva, S., Markova, N. Combined immunomodulating effects of BCG and Lentinan after intranasal application in guinea pigs. International Immunopharmacology. 2005 Apr;5 (4):795-803.

Michailova, L, Kussovski, V., Radoucheva, T., Jourdanova, M., Berger, W., Rinder, H., Markova, N. Morphological variability and cell wall deficiency of Mycobacterium tuberculosis in “heteroresistant” strains isolated from newly diagnosed tuberculosis patients. The International Journal of Tuberculosis and Lung Disease. 2005 Aug;9 (8):907-14.

Bonovska, M., Tzvetkov, Y., Najdenski, H., Bachvarova, Y. PCR for detection of Mycobacterium tuberculosis in experimentally infected dogs. J. Vet. Med., 2005, 52(10), 5-8.

Ivanova, A., B. Mikhova, H. Najdenski, I. Tzvetkova, I.Kostova. Antimicrobial and cytotoxic activity of Ruta graveolens. Fitoterapia, 76, 2005, 344-347.

Славова-Азманова, Н. Х. Найденски, Антибиотична резистентност – молекулярни механизми и механизми за нейното преодоляване. – Инфекциозни заболявания, 36, 2005, 2, 3-22.

Najdenski, H., Golkocheva, E., Kussovski, V., Ivanova, E., Manov, V., Iliev M., Vesselinova, A., Bengoechea, J.A., Skurnik, M. Experimental pig yersiniosis to assess attenuation of Yersinia enterocolitica O:8 mutant strains. FEMS Immunol. Med. Microbiol., 47, 425-435, 2006.

Yordanov, M., Golkocheva, E., Najdenski, H. Modulation of complement activity in vitro and in vivo by Yersinia wild and mutant strains. Folia Microbiol., 51, 27-32, 2006.

Mokrousov, I., Jiao,W.W., Valcheva, V., Vyazovaya, A., Otten, T., Ly, H. M., Lan, N. N., Limeschenko, E., Markova, N., Vyshnevskiy, B., Shen, A.D., Narvskaya O. Rapid Detection of the Mycobacterium tuberculosis Beijing genotype and its ancient and modern sublineages by IS6110-based inverse PCR. J. Clin. Microbiol., 44: 2851–2856, 2006.

Mokrousov, I., Jiao, W.W., Sun, G.Z., Liu, J.W., Valcheva, V., Li, M., Narvskaya, O., Shen, A.D. Evolution of drug resistance in different sublineages of Mycobacterium tuberculosis Beijing genotype. Antimicrob. Agents and Chemotherapy, 50, 2820-2823, 2006.

Ignatova, M., Voccia, S., Gilbert B., Markova, N., Cossement, D., Gouttebaron, R., Jérôme, R and Ch. Jérôme .Combination of electrografting and atom-transfer radical polymerization for making the stainless steel surface antibacterial and protein antiadhesive. Langmuir, 22, 255 –262, 2006.

Ignatova, M., Starbova, K., Markova, N., Manolova, N., Rashkov, I. Electrospun nano-fibre mats with antibacterial properties from quaternised chitosan and poly (vinyl alcohol). Carbohydrate Research, 341, 2098-2107, 2006.

Golkocheva, E., Stoilov, R., Najdenski, H. Serological findings in blood sera of patients with Yersinia-triggered arthritis. Clin. Microbiol. Infect., 12 (s4), P1739, 2006.

Carballeira, N., Ivanova, A., Wagner, K., O’Neill, R., H. Najdenski, Tsvetkova, I., Stefanov, K. Fatty acid composition of Staphylococcus epidermis strain isolated from lake Pomorie in Bulgaria. Compt. Rend. Acad. Bulg. Sci., 59, 8, 849-852, 2006.

Konakchiev, A., Todorova, M., Mikhova, B., Vitkova, A., Najdenski, H., Duddeck, H. Chemical composition and antimicrobial activity of the essential oil from two Achillea collina Becker. Compt. Rend. Acad. Bulg. Sci., 59, 5, 505-510, 2006.

Голкочева, Е., Стоилов, Р., Найденски, Х. Реактивен артрит асоцииран с Yersinia enterocolitica – патогенеза и диагноза, Ревматология, 3, 2006.

Slavova-Azmanova, N. Purification and biochemical characterization of UMP kinaze from Streptococcus pneumoniae. Compt. Rend. Acad. Bulg. Sci., 59, 859-864, 2006.



Хайдушка И , Мурджева, М., Вълчева, В., Маркова, Н. Адхезивни свойства, вирулентност и физиологични характеристики на клинични метицилин- резистентни изолати Staphylococcus aureus. Инфектология, 3, 2006.


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