Supplementary Fig. 1 Multiple alignment of EpFPS with the following FPSs of other organisms:Ganoderma lucidum ( GlFPS, ACB37020), Homo sapiens (HsFPS, NP_001995),Drosophila melanogaster ( DmFPS, CAA08919)and Escherichia coli ( EcFPS, BAA00599). Identical and conserved amino acid residues are denoted by black and gray backgrounds, respectively. The five conserved domains of prenyltransferases are boxed and numbered. The highly conserved aspartate-rich motifs (DDXX(XX)D) is present in domains II and V. The highly conserved amino acid sequences used for primers are marked with *.
Supplementary Fig. 2 A phylogenetic tree of FPSs from various organisms by MEGA4.0 from Clustal X alignment. The neighbor-joining method was used to construct the tree. The FPSs used in phylogenetic tree analysis were from Hevea brasiliensis(AAM98379),Malus x domestica(AAM08927),Gentiana lutea(BAA88844), Euphorbia pekinensis (FJ755465), Gossypium arboreum(CAA72793), Mentha x piperita (AAK63847), Panax ginseng(AAY87903), Matricaria recutita (ABS11699), Artemisia annua(AAD17204), Centella asiatica(AAV58896), Ganoderma lucidum (ACB37020), Lactarius chrysorrheus(BAD15361),Mucor circinelloides(CAD42869), Neurospora crassa (XP_961541), Drosophila melanogaster(CAA08919), Aedes aegypti(XP_001663796), Culex quinquefasciatus(XP_001841747), Dendroctonus jeffreyi (AAX78435), Myzus persicae(ABY19312), Escherichia coli(BAA00599), Micrococcus luteus (BAA25265 ),Macrococcus caseolyticus(YP_002560571), Clostridium thermocellum (YP_001037259), Geobacillus stearothermophilus (BAA02551).