|Dr. Duan has been working in the field of plant biotechnology for many years. Currently Dr. Duan is the molecular team 2 leader in J. R. Simplot company's Plant Sciences Business, working on research and development of a number of potato agronomic and consumer traits using intragenic and cisgenic technology. Dr. Duan obtained his PhD degree from the University of Connecticut, where he was a key developer of the Gene-Deletor technology in Dr. Yi Li's lab. Then he did his postdoctoral research with Dr. Mary Schuler at the University of Illinois, as a key player in an NSF 2010 project, functional genomics of plant P450 genes. Dr. Duan has more than 25 publications appearing in journals such as Plant Physiology, Arch. in Biochemistry and Biophysics, Plant Biotechnology Journal, PROTEINS, RNA, Photochemistry Reviews and Transgenic Research. In this talk he will present his recent work and his point of view on genetic engineering.
Li, Y and Duan, H (2011) Molecular approaches for transgene containment and their potential applications in horticultural crops. In: Transgenic horticultural crops: challenges and opportunities. Mou. B (ed.) CRC Press (in press)
Chen, Y., Lu, L., Yang, X., Duan, H., Deng, W., McAvoy, R., Zhao, D., Smith, W., Thammina, C., von Bodman, S. and Li, Y. (2008) Progress report of production of sterile cultivars of euonymus. Acta Horticulturae. 769: 232-239.
Rupasinghe, S., Duan, H., Frericks-Schmidt, HL., Berthold, DA., Rienstra, CM and Schuler, MA (2007) High-yield expression and purification of isotopically labeled cytochrome P450 monooxygenases for solid-state NMR spectroscopy. Biochimica et Biophysica Acta 1768: 3061-3070
Luo, K., Duan, H., Zhao, D., Zheng, X., Deng, W., Chen, Y., McAvoy, R., Jiang, X., Wu, Y., Pei, Y and Li, Y (2007) A highly efficient system to delete all functional transgenes from pollen of tobacco plants. Acta Horticulturae. 764: 21-31.
Rupasinghe, S., Duan, H and Schuler, MA (2007) Molecular definitions of fatty acid hydroxylase in Arabidopsis thaliana. PROTEINS: Structure, Function, and Bioinformatics 68: 279-293
Zheng, X., Deng, W., Luo, K., Duan, H., Chen, Y., McAvoy, R., Song, S., Pei, Y and Li, Y (2007) The cauliflower mosaic virus (CaMV) 35S promoter sequence alters the level and patterns of activity of adjacent tissue- and organ-specific gene promoters. Plant Cell Reports. 26: 1195-1203
Luo, KM., Duan, H., Zhao, DG., Zheng X, Deng W, Yongqin Chen, Y., Stewart, CN., McAvoy, R., Jiang, X., Wu, Y., He, A., Pei, Y and Li, Y (2007) ‘GM-gene-deletor’: fused loxP-FRT recognition sequences dramatically improve efficiency of FLP or Cre recombinase on transgene excision from pollen and seed of tobacco plants. Plant Biotech. J. 5: 263-274
Duan, H and Schuler, MA (2006) Heterologous expression and strategies for encapsulation of membrane-localized plant P450s. Phytochem. Reviews 5: 507-523
Schuler, MA., Duan, H., Metin, B and Ali, S (2006) Arabidopsis P450s through the looking glass: a window on plant biochemistry. Phytochem. Reviews 5: 205-237
Chen, Y., Lu, L., Deng, W., Yang, X., McAvoy, R., Zhao, D., Pei, Y., Luo, K., Duan, H., Y. Li (2006) In vitro regeneration and Agrobacterium-mediated genetic transformation of Euonymus alatus. Plant Cell Reports. 25: 1043-1051
Duan, H., Pei, Y., McAvoy, RJ., Zhao, D., Chen, Y., Luo, K., Smith RW and Li, Y(2006): Auxin, cytokinin and abscisic acid: biosynthetic and catabolic genes and their potential applications in horticultural crops. J. of Crop Improvement. 18: 347-364
Li, Y., Cheng, Z., Smith, RW., Ellis, D., Chen, Y., Zheng, X., Pei, Y., Luo K., McAvoy, R., Duan, H., Thammina, C and Zhao, D. (2006) Gene transfer approaches to neutralize invasiveness of exotic ornamental plants. J. of Crop Improvement. 17: 279-301
Xiong, A., Yao, QH., Peng, RH., Duan, H., Li, X., Fan, HQ., Cheng, ZM and Li, Y (2006) PCR-based accurate synthesis of long DNA sequences. Nature Protocols 1: 791-797
Duan, H., Huang, MY., Palacio, K and Schuler, MA (2005) Variations in CYP74B2 (hydroperoxide lyase) gene expression differentially affect hexenal signaling in the Columbia and Landsberg ecotypes of Arabidopsis Plant Physiol. 139: 1529-44
Duan, H., and Schuler, MA. (2005) Differential expression and evolution of Arabidopsis CYP86A subfamily. Plant Physiol. 137: 1067-81.